EGFRviii Antikörper (AA 1-50)

Produktnummer ABIN742035

Dieser Anti-EGFRviii Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von EGFRviii in WB, FACS, IF (cc), IF (p), ELISA, IHC (fro) und IHC (p). Geeignet für Human. Dieses Primary Antibody wurde in 9+ Publikationen zitiert.

Kurzübersicht für EGFRviii Antikörper (AA 1-50) (ABIN742035)

Target: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGFRviii Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-EGFRviii Antikörper

Bindungsspezifität: AA 1-50

Kreuzreaktivität: Huhn, Human, Maus

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EGFRvIII

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-EGFRviii Antikörper (ABIN742035)

Khelwatty, Essapen, Bagwan, Green, Seddon, Modjtahedi: "The impact of co-expression of wild-type EGFR and its ligands determined by immunohistochemistry for response to treatment with cetuximab in patients with metastatic colorectal cancer." in: Oncotarget, Vol. 8, Issue 5, pp. 7666-7677, (2018) (PubMed).

Wei, Cui, Zhou, Fang, Tan, Chen, Yang, Liu, Kang: "F25P preproinsulin abrogates the secretion of pro-growth factors from EGFRvIII cells and suppresses tumor growth in an EGFRvIII/wt heterogenic model." in: Cancer letters, Vol. 380, Issue 1, pp. 1-9, (2016) (PubMed).

Wang, Kang, Wang-Gou, Huang, Feng, Li: "EGFL7 is an intercellular EGFR signal messenger that plays an oncogenic role in glioma." in: Cancer letters, Vol. 384, pp. 9-18, (2016) (PubMed).

Liu, Han, Dong, Tan, Li, Zhao, Xie, Ju, Wang, Zhao, Zheng, Wang, Su, Fang, Fu, Jiang, Liu, Li, Kang, Ren: "EGFRvIII/integrin β3 interaction in hypoxic and vitronectinenriching microenvironment promote GBM progression and metastasis." in: Oncotarget, Vol. 7, Issue 4, pp. 4680-94, (2016) (PubMed).

Wehrenberg-Klee, Redjal, Leece, Turker, Heidari, Shah, Mahmood: "PET imaging of glioblastoma multiforme EGFR expression for therapeutic decision guidance." in: American journal of nuclear medicine and molecular imaging, Vol. 5, Issue 4, pp. 379-89, (2015) (PubMed).

Jørgensen, Bæk, Varming: "Potentials and capabilities of the Extracellular Vesicle (EV) Array." in: Journal of extracellular vesicles, Vol. 4, pp. 26048, (2015) (PubMed).

Agnes, Broome, Wang, Verma, Lavik, Basilion: "An optical probe for noninvasive molecular imaging of orthotopic brain tumors overexpressing epidermal growth factor receptor." in: Molecular cancer therapeutics, Vol. 11, Issue 10, pp. 2202-11, (2012) (PubMed).

Details zu EGFRviii

Target: EGFRviii (EGFRv3) (EGFR vIII (EGFRv3))

Hintergrund:

Synonyms: EGFR vIII, EGFRvIII, Epidermal growth factor receptor variant type _, epidermal growth factor receptor isoform a variant, ERBB, HER1, ERBB1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR

Background: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.

Gen-ID: 1956

UniProt: Q59FL8