CD72 Antikörper (FITC)

Produktnummer ABIN7836576

Dieser Maus Monoklonal Antikörper detektiert spezifisch CD72 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für CD72 Antikörper (FITC) (ABIN7836576)

Target: CD72 (CD72 Molecule (CD72))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD72 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 10-1-D2

Produktdetails anti-CD72 Antikörper (FITC)

Verwendungszweck: Mouse monoclonal antibody FITC labled to CD72.1

Spezifität: Recognizes mouse CD72.1

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: DBA/2 mouse spleen cells

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD72

Target: CD72 (CD72 Molecule (CD72))

Andere Bezeichnung: CD72

Hintergrund: B-cell differentiation antigen CD72, Lyb-2, CD72

Gen-ID: 12517

Pathways: BCR Signaling