CD70 Antikörper

Produktnummer ABIN7836565

Der Ratte Monoklonal anti-CD70 Antikörper wird verwendet zum Nachweis von CD70 in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für CD70 Antikörper (ABIN7836565)

Target: CD70 (CD70 Molecule (CD70))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CD70 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: FR70

Produktdetails anti-CD70 Antikörper

Verwendungszweck: Rat monoclonal antibody to CD70

Spezifität: Recognizes mouse CD70

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: BALB/c mouse B lymphoma A20.2J

Anwendungsinformationen

Applikationshinweise: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu CD70

Target: CD70 (CD70 Molecule (CD70))

Andere Bezeichnung: CD70

Hintergrund: CD27L, CD27LG, TNFSF7, CD70 antigen, CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7, CD70

Gen-ID: 21948

Pathways: Cancer Immune Checkpoints