CD55 Antikörper (PE-Cy5)

Produktnummer ABIN7836469

Der Maus Monoklonal anti-CD55 Antikörper wird verwendet zum Nachweis von CD55 in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für CD55 Antikörper (PE-Cy5) (ABIN7836469)

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD55 Antikörper ist konjugiert mit PE-Cy5

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 143-30

Produktdetails anti-CD55 Antikörper (PE-Cy5)

Verwendungszweck: Mouse monoclonal antibody PE/Cy5 labled to CD55

Spezifität: Recognizes human CD55

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD55.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Andere Bezeichnung: CD55

Hintergrund: CR, DAF, Complement decay-accelerating factor, CD antigen CD55

Gen-ID: 1604

UniProt: P08174

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity