Integrin Alpha2b Antikörper (FITC)

Produktnummer ABIN7836295

Der Maus Monoklonal anti-Integrin Alpha2b Antikörper wird verwendet zum Nachweis von Integrin Alpha2b in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für Integrin Alpha2b Antikörper (FITC) (ABIN7836295)

Target: Integrin Alpha2b (CD41)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Integrin Alpha2b Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: HIP8

Produktdetails anti-Integrin Alpha2b Antikörper (FITC)

Verwendungszweck: Mouse monoclonal antibody FITC labled to CD41

Spezifität: Recognizes human CD41

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD41.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu Integrin Alpha2b

Target: Integrin Alpha2b (CD41)

Andere Bezeichnung: ITGA2B

Hintergrund: GP2B, ITGAB, Integrin alpha-IIb, GPalpha IIb, GPIIb, Platelet membrane glycoprotein IIb, CD41

Gen-ID: 3674

UniProt: P08514

Pathways: Integrin Complex