CD35 Antikörper (PE)

Produktnummer ABIN7836199

Der Maus Monoklonal anti-CD35 Antikörper wird verwendet zum Nachweis von CD35 in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für CD35 Antikörper (PE) (ABIN7836199)

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD35 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: E11

Produktdetails anti-CD35 Antikörper (PE)

Verwendungszweck: Mouse monoclonal antibody PE labled to CD35

Spezifität: Recognizes human CD35

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD35.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD35

Target: CD35 (CR1) (Complement Component Receptor 1 (CD35) (CR1))

Andere Bezeichnung: CR1

Hintergrund: C3BR, Complement receptor type 1, C3b/C4b receptor, CD antigen CD35

Gen-ID: 1378

UniProt: P17927

Pathways: Komplementsystem, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Endopeptidase Activity