CCR5 Antikörper

Produktnummer ABIN7835902

Der Ratte Monoklonal anti-CCR5 Antikörper wird verwendet zum Nachweis von CCR5 in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für CCR5 Antikörper (ABIN7835902)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser CCR5 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: HEK-1-85a

Produktdetails anti-CCR5 Antikörper

Verwendungszweck: Rat monoclonal antibody to CD195

Spezifität: Recognizes human CD195

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: CHO cells transfected with human CCR5

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Details zu CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Andere Bezeichnung: CCR5

Hintergrund: CMKBR5, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195

Gen-ID: 1234

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size