CCR5 Antikörper (AA 1-22)

Produktnummer ABIN1558080

Zitiert in 3+ Publikationen. Der Maus Monoklonal anti-CCR5 Antikörper (Klon T21-8) (ABIN1558080) detektiert spezifisch CCR5 in WB, ELISA, FACS, IP und ICC. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für CCR5 Antikörper (AA 1-22) (ABIN1558080)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CCR5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Klon: T21-8

Produktdetails anti-CCR5 Antikörper

Bindungsspezifität: AA 1-22

Verwendungszweck: Anti-Hu CD195 Purified

Kreuzreaktivität (Details): Human

Produktmerkmale: The mouse monoclonal antibody T21/8 recognizes an extracellular epitope on the N-teminus of CD195, an approximately 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: CCR5 peptide (Met1-Lys22) KLH conjugate

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Western blotting: Do not boil cell lysates prior to SDS-PAGE.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-CCR5 Antikörper (ABIN1558080)

Monde, Maeda, Tanaka, Harada, Yusa: "Gp120 V3-dependent impairment of R5 HIV-1 infectivity due to virion-incorporated CCR5." in: The Journal of biological chemistry, Vol. 282, Issue 51, pp. 36923-32, (2007) (PubMed).

Hüttenrauch, Pollok-Kopp, Oppermann: "G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers." in: The Journal of biological chemistry, Vol. 280, Issue 45, pp. 37503-15, (2005) (PubMed).

Pollok-Kopp, Schwarze, Baradari, Oppermann: "Analysis of ligand-stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite-specific antibodies." in: The Journal of biological chemistry, Vol. 278, Issue 4, pp. 2190-8, (2003) (PubMed).

Details zu CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Andere Bezeichnung: CD195

Hintergrund: C-C motif chemokine receptor 5,CD195 / CCR5 (also known as CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 alpha, MIP-1 beta, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 / CCR5 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.,CKR5, CCR5, CCCKR5, CMKBR5, IDDM22, CC-CKR-5

Gen-ID: 1234

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size