CD40 Ligand Antikörper (FITC)

Produktnummer ABIN7835791

Der Armenischer Hamster Monoklonal anti-CD40 Ligand Antikörper wird verwendet zum Nachweis von CD40 Ligand in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für CD40 Ligand Antikörper (FITC) (ABIN7835791)

Target: CD40 Ligand (CD40LG)

Reaktivität: Maus

Wirt: Armenischer Hamster

Klonalität: Monoklonal

Konjugat: Dieser CD40 Ligand Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: MR1

Produktdetails anti-CD40 Ligand Antikörper (FITC)

Verwendungszweck: Armenian Hamster monoclonal antibody FITC labled to CD154

Spezifität: Recognizes mouse CD154

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Activated mouse Th1 clone D1.6

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu CD40 Ligand

Target: CD40 Ligand (CD40LG)

Andere Bezeichnung: CD40LG

Hintergrund: CD40L, TNFSF5, TRAP, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154

Gen-ID: 21947

Pathways: NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints