IL3RA Antikörper (PE-Cy7)

Produktnummer ABIN7835704

Der Maus Monoklonal anti-IL3RA Antikörper wird verwendet zum Nachweis von IL3RA in Proben von Human. Er wurde validiert für FACS und IF.

Kurzübersicht für IL3RA Antikörper (PE-Cy7) (ABIN7835704)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL3RA Antikörper ist konjugiert mit PE-Cy7

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 6H6

Produktdetails anti-IL3RA Antikörper (PE-Cy7)

Verwendungszweck: Mouse monoclonal antibody PE/Cy7 labled to CD123

Spezifität: Recognizes human CD123

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD123.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Andere Bezeichnung: IL3RA

Hintergrund: IL3R, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, CD antigen CD123

Gen-ID: 3563

UniProt: P26951

Pathways: JAK-STAT Signalweg