Rekombinanter EGFR Antikörper (pTyr1173)

Produktnummer ABIN7828180

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch EGFR in WB und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter EGFR Antikörper (pTyr1173) (ABIN7828180)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser EGFR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Klon: A825

Produktdetails anti-EGFR Antikörper

Bindungsspezifität: pTyr1173

Verwendungszweck: Recombinant Phospho-EGFR (Tyr1173) Monoclonal Antibody

Aufreinigung: Protein A purified

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: WB 1:1000,IF 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Haltbarkeit: 12 months

Details zu EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Andere Bezeichnung: EGFR

Hintergrund: PIG,HER,Proto-oncogene c-ErbB,Receptortyrosine-protein kinase erbB,Receptor tyrosine-protein kinase erbB,NISBD,EGFR,ERBB,ERBB1,HER1,NISBD2,PIG61,mENA,ErbB-1,Receptortyrosine-protein kinase erbB-1,Epidermal growth factor receptor,Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1, NISBD2,epidermal growth factor receptor,ERBB,ERBB1,HER1,mENA,NISBD2,PIG61,Avian erythroblastic leukemia viral (v erb b) oncogene homolog,Cell growth inhibiting protein 40,Cell proliferation inducing protein 61,EGF R,Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog),Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian),ErbB1,ERBB,erb-b2 receptor tyrosine kinase 1,Errp,Oncogen ERBB,Receptor tyrosine protein kinase ErbB 1,SA7,Species antigen 7,Urogastrone,v-erb-b Avian erythroblastic leukemia viral oncogen homolog,wa2,Wa5,The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand bindingresults in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation.Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the activestate enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domainof PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling. Phosphorylation of EGFR at Tyr1045creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation. TheGRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide adocking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specificserine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylatedby CaM kinase II, mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molekulargewicht:

Calculated MW: 134 kDa

Observed MW: 170 kDa The actual band is not consistent with the expectation.

UniProt: P00533

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteine