Rekombinanter Cyclin B1 Antikörper

Produktnummer ABIN7828138

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch Cyclin B1 in WB, IF und IP. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter Cyclin B1 Antikörper (ABIN7828138)

Target: Cyclin B1 (CCNB1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Klon: A546

Produktdetails anti-Cyclin B1 Antikörper

Verwendungszweck: Recombinant Cyclin B1 Monoclonal Antibody

Aufreinigung: Protein A purified

Immunogen: Recombinant human Cyclin B1 fragment

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000,IF 1:50,IP 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Haltbarkeit: 12 months

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1

Hintergrund: G2 mitotic specific cyclin B,G2/mitotic-specific cyclin-B,Cyclin B,CCNB,CCNB1,Cyclin B1,CCNB 1,G2 mitotic specific cyclin B1,G2/mitotic-specific cyclin-B1,Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molekulargewicht:

Calculated MW: 55 kDa

Observed MW: 55 kDa

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase