VEGFA Antikörper (CF®740)

Produktnummer ABIN7661853

Dieses Anti-VEGFA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von VEGFA in IHC und IHC (fp). Geeignet für Human, Ratte, Maus, Hund und Kaninchen.

Kurzübersicht für VEGFA Antikörper (CF®740) (ABIN7661853)

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Reaktivität: Human, Ratte, Maus, Hund, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VEGFA Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: VEGF-1063

Produktdetails anti-VEGFA Antikörper (CF®740)

Verwendungszweck: VEGF-A(VEGF/1063), CF740 conjugate

Produktmerkmale: This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human VEGF189 recombinant protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGF-A

Hintergrund:

Synonyms: MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA

Gene Symbol: VEGFA

Tissue Expression: Endothelial cells

Molekulargewicht: 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)

Gen-ID: 7422

UniProt: P15692

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling