TGFA Antikörper (AA 34-43) (CF®740)

Produktnummer ABIN7661057

Der Maus Monoklonal Anti-TGFA-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, TGFA in Proben von Human, Kaninchen und Zebrafisch (Danio rerio) zu detektieren.

Kurzübersicht für TGFA Antikörper (AA 34-43) (CF®740) (ABIN7661057)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reaktivität: Human, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFA Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: TG86

Produktdetails anti-TGFA Antikörper (CF®740)

Bindungsspezifität: AA 34-43

Verwendungszweck: TGFalpha(TG86), CF740 conjugate

Produktmerkmale: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A 10-amino acid synthetic peptide (aa34-43, PPVAAAVVSH) from human TGF?.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-paraffin 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Jurkat or Ramos cells. Testicular Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGF-Alpha

Hintergrund:

Synonyms: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Gene Symbol: TGFA

Molekulargewicht: 6 kDa

Gen-ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway