Topoisomerase II alpha Antikörper (AA 1352-1493) (CF®740)

Produktnummer ABIN7660930

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Topoisomerase II alpha in WB, IHC, IF und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1352-1493) (CF®740) (ABIN7660930)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: TOP2A-1362

Produktdetails anti-Topoisomerase II alpha Antikörper (CF®740)

Bindungsspezifität: AA 1352-1493

Verwendungszweck: Topoisomerase II alpha (Proliferation & Drug-Resistance Marker) (TOP2A/1362), CF740 conjugate

Produktmerkmale: This antibody recognizes a 170 kDa protein, which is identified as topoisomerase IIα. It shows no cross-reaction with topoisomerase IIβ or topoisomerase I. Topo IIα plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIα is also implicated in drug resistance of tumor cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa Cells or Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: Topoisomerase-II-Alpha

Hintergrund:

Synonyms: ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, DNA Topoisomerase2, TOP2A, Topoisomerase DNA II alpha 170 kDa, TP2A

Gene Symbol: TOP2A

Molekulargewicht: 170 kDa

Gen-ID: 7153

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases