Thymidine Phosphorylase Antikörper (CF®740)

Produktnummer ABIN7660713

Dieses Anti-Thymidine Phosphorylase-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Thymidine Phosphorylase in WB, IHC und IHC (fp). Geeignet für Human, Ratte und Maus.

Kurzübersicht für Thymidine Phosphorylase Antikörper (CF®740) (ABIN7660713)

Target: Thymidine Phosphorylase (TYMP)

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Thymidine Phosphorylase Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: P-GF-44C

Produktdetails anti-Thymidine Phosphorylase Antikörper (CF®740)

Verwendungszweck: Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker) (P-GF.44C), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin) 1-2 μg/mL. Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HUVEC cells. Breast, Bladder, Lung or Kaposi tumors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Thymidine Phosphorylase

Target: Thymidine Phosphorylase (TYMP)

Andere Bezeichnung: PD-ECGF

Hintergrund:

Synonyms: ECGF, ECGF1, Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PD-ECGF, PDECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine phosphorylase, TP, Tymp

Gene Symbol: TYMP

Molekulargewicht: 55 kDa

Gen-ID: 1890

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2