Retinoblastoma 1 Antikörper (CF®740)

Produktnummer ABIN7659622

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Retinoblastoma 1 in . Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Retinoblastoma 1 Antikörper (CF®740) (ABIN7659622)

Target: Retinoblastoma 1 (RB1)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Retinoblastoma 1 Antikörper ist konjugiert mit CF®740

Applikation: Bitte anfragen

Klon: RB1-1754

Produktdetails anti-Retinoblastoma 1 Antikörper (CF®740)

Verwendungszweck: Rb1 (Tumor Suppressor Protein) (RB1/1754), CF740 conjugate

Produktmerkmale: This antibody recognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Rb1 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Andere Bezeichnung: Rb1

Hintergrund:

Synonyms: OSRC, Osteosarcoma, p105-Rb, PP105, pp110, pRb, Prepro retinoblastoma associated protein, RB1, Retinoblastoma 1, Retinoblastoma-associated protein

Gene Symbol: RB1

Molekulargewicht: 105 kDa

Gen-ID: 5925

UniProt: P06400

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagie