Prostate Specific Antigen Antikörper (CF®405S)

Produktnummer ABIN7659236

Der Maus Monoklonal Anti-Prostate Specific Antigen-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Prostate Specific Antigen in Proben von Human und Cat zu detektieren.

Kurzübersicht für Prostate Specific Antigen Antikörper (CF®405S) (ABIN7659236)

Target: Prostate Specific Antigen (PSA)

Reaktivität: Human, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Prostate Specific Antigen Antikörper ist konjugiert mit CF®405S

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 1A7

Produktdetails anti-Prostate Specific Antigen Antikörper (CF®405S)

Verwendungszweck: Prostate Specific Antigen (PSA)(1A7), CF405S conjugate

Produktmerkmale: This antibody recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. This MAb makes an excellent pair with MAb A67-B/E3 for PSA tests.

Immunogen: PSA (p30) from human sperm plasma

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: PC-3 or LNCaP cell lysates (WB). Prostate tissue (IHC). LNCaP cells (IF).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Andere Bezeichnung: Prostate Specific Antigen

Hintergrund:

Synonyms: Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

Gene Symbol: KLK3

Tissue Expression: Prostate

Molekulargewicht: 33-34 kDa

Gen-ID: 354

UniProt: P07288

Pathways: Komplementsystem