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PODXL Antikörper (Extracellular Domain) (CF®740)

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PODXL in WB, IHC, FACS, IF und IHC (fp). Er zeigt eine Reaktivität gegenüber Human, Ratte und Kaninchen.
Produktnummer ABIN7658743

Kurzübersicht für PODXL Antikörper (Extracellular Domain) (CF®740) (ABIN7658743)

Target

Alle PODXL Antikörper anzeigen
PODXL (Podocalyxin-Like (PODXL))

Reaktivität

  • 106
  • 27
  • 14
  • 11
  • 1
  • 1
Human, Ratte, Kaninchen

Wirt

  • 68
  • 55
  • 1
Maus

Klonalität

  • 70
  • 54
Monoklonal

Konjugat

  • 67
  • 10
  • 7
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Dieser PODXL Antikörper ist konjugiert mit CF®740

Applikation

  • 74
  • 65
  • 34
  • 33
  • 32
  • 24
  • 20
  • 18
  • 12
  • 9
  • 5
  • 4
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon

3D3
  • Bindungsspezifität

    • 23
    • 16
    • 9
    • 8
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain

    Verwendungszweck

    Podocalyxin(3D3), CF740 conjugate

    Produktmerkmale

    Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is highly on kidney podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    Immunogen

    A recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain of human podocalyxin

    Isotyp

    IgG1
  • Applikationshinweise

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Immunoprecipitation 1-2 μg/500 μg protein. Optimal dilution for a specific application should be determined by user

    Kommentare

    Positive Control: HeLa, Raji, Jurkat cells, Angiosarcoma, Breast, Prostate, Liver, Pancreatic, Kidney

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    0.1 mg/mL

    Buffer

    PBS, 0.1 % rBSA, 0.05 % azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Protect from light

    Lagerung

    4 °C

    Informationen zur Lagerung

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    Haltbarkeit

    24 months
  • Target

    PODXL (Podocalyxin-Like (PODXL))

    Andere Bezeichnung

    Podocalyxin

    Hintergrund

    Synonyms: PODXL, PDXL, GCTM-2, Gp200, PCLP1, Pcx, Podocalyxin-like protein 1

    Gene Symbol: PODXL

    Tissue Expression: Kidney

    Molekulargewicht

    165-170 kDa

    Gen-ID

    5420

    UniProt

    O00592

    Pathways

    Tube Formation
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