PLAP Antikörper (CF®740)

Produktnummer ABIN7658621

Der Maus Monoklonal Anti-PLAP-Antikörper wurde für WB, IHC und IHC (fp) validiert. Er ist geeignet, PLAP in Proben von Human zu detektieren.

Kurzübersicht für PLAP Antikörper (CF®740) (ABIN7658621)

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLAP Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: GM022

Produktdetails anti-PLAP Antikörper (CF®740)

Verwendungszweck: PLAP (Placental Alkaline Phosphatase)(GM022), CF740 conjugate

Produktmerkmale: Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human PLAP protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.25-0.5 μg/mL. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HepG2 cells. Placenta or seminoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Andere Bezeichnung: PLAP

Hintergrund:

Synonyms: Alkaline phosphatase placental type|Alkaline phosphatase Regan Isozyme|ALP|Alp1|ALPP|Germ-cell alkaline phosphatase|nagao Isozyme|PALP|Placental alkaline phosphatase 1|placental heat-stable alkaline phosphatase|PLAP-1|PLAP1

Gene Symbol: ALPP

Tissue Expression: Placenta

Molekulargewicht: 70 kDa

Gen-ID: 250

UniProt: P05187