Perforin 1 Antikörper (AA 413-552)

Produktnummer ABIN7658546

Der Maus Monoklonal Anti-Perforin 1-Antikörper wurde für validiert. Er ist geeignet, Perforin 1 in Proben von Human zu detektieren.

Kurzübersicht für Perforin 1 Antikörper (AA 413-552) (ABIN7658546)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Perforin 1 Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Klon: PRF1-2468

Produktdetails anti-Perforin 1 Antikörper

Bindungsspezifität: AA 413-552

Verwendungszweck: Perforin-1 (Pore Forming Protein) (Apoptosis Marker) (PRF1/2468)

Produktmerkmale: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8 T-cells, natural killer cells and some CD4 T-cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Perforin-1 protein fragment (around aa 413-552)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HDLM-2 Cells. Spleen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Andere Bezeichnung: Perforin-1

Hintergrund:

Synonyms: Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein, PRF1 (pore forming protein 1), Perforin-1, PFP, PGFL, PIGF, PIGF-2, PLGF

Gene Symbol: PRF1

Tissue Expression: NK cells|T-cells

Molekulargewicht: 75 kDa

Gen-ID: 5551

UniProt: P14222

Pathways: Apoptose, Caspase Kaskade in der Apoptose