NME1 Antikörper (CF®740)

Produktnummer ABIN7658062

Dieses Maus Monoklonal-Antikörper erkennt spezifisch NME1 in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NME1 Antikörper (CF®740) (ABIN7658062)

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NME1 Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB)

Klon: CPTC-NME1-2

Produktdetails anti-NME1 Antikörper (CF®740)

Verwendungszweck: NME1 / nm23-H1 / NDPK-A (Suppressor of Metastasis)(CPTC-NME1-2), CF740 conjugate

Produktmerkmale: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP-K). In humans, NDP kinase A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human C-Myc promotor. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human NME1 protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: HepG2 and HeLa whole cell lysates, heart, brain, placenta and lung carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu NME1

Target: NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))

Andere Bezeichnung: NDPK-A

Hintergrund:

Synonyms: AWD, GAAD, Granzyme A activated DNase, Metastasis inhibition factor NM23, NB, NBS, NDP kinase A, NM23 long variant, included, NM23H1B, NME/NM23 nucleoside diphosphate kinase 1, NME1-NME2 spliced read-through transcript, included, Non-metastatic protein 23, homolog 1, Nucleoside diphosphate kinase A, Tumor metastatic process-associated protein

Gene Symbol: NME1

Molekulargewicht: 23 kDa

Gen-ID: 4830

UniProt: P15531

Pathways: Apoptose, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process