Rekombinanter Neurofilament Antikörper (CF®488A)

Produktnummer ABIN7657881

Der CF®488A-konjugierte Maus Monoklonal anti-Neurofilament Antikörper (Klon rNF421) (ABIN7657881) detektiert spezifisch Neurofilament in FACS, IHC, IHC (fp) und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Huhn und Schwein.

Kurzübersicht für Rekombinanter Neurofilament Antikörper (CF®488A) (ABIN7657881)

Target: Neurofilament

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Huhn, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Neurofilament Antikörper ist konjugiert mit CF®488A

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Klon: RNF421

Produktdetails anti-Neurofilament Antikörper (CF®488A)

Verwendungszweck: Neurofilament (NF-H) (Neuronal Marker) (rNF421), CF488A conjugate

Produktmerkmale: This MAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human NEFH protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1.0 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Brain, Neuroblastoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Neurofilament

Target: Neurofilament

Hintergrund:

Synonyms: NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200 kDa, Neurofilament Triplet H Protein, NF-H, NF200

Gene Symbol: NEFH

Tissue Expression: Neuroendocrine cells|Neurons

Molekulargewicht: 200 kDa

Gen-ID: 4744

UniProt: P12036