Neurofilament Antikörper

Produktnummer ABIN3023931

Der Maus Monoklonal anti-Neurofilament Antikörper wird verwendet zum Nachweis von Neurofilament in Proben von Human. Er wurde validiert für WB und IHC (p).

Kurzübersicht für Neurofilament Antikörper (ABIN3023931)

Target: Neurofilament

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Neurofilament Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: SPM563

Produktdetails anti-Neurofilament Antikörper

Verwendungszweck: Anti-Neurofilament Antibody (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant protein was used as the immunogen for this anti-Neurofilament antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The optimal dilution of the anti-Neurofilament antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the anti-Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Neurofilament

Target: Neurofilament

Hintergrund: This mAb reacts with a 200 kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.

UniProt: P12036