Neurofilament Antikörper

Produktnummer ABIN3025341

Der Maus Monoklonal anti-Neurofilament Antikörper wird verwendet zum Nachweis von Neurofilament in Proben von Human, Ratte und Maus. Er wurde validiert für FACS, WB, IF und IHC (p).

Kurzübersicht für Neurofilament Antikörper (ABIN3025341)

Target: Neurofilament

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Neurofilament Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: NF421

Produktdetails anti-Neurofilament Antikörper

Verwendungszweck: Neurofilament Antibody / Heavy (azide and preservative free)

Aufreinigung: Protein G purified Neurofilament antibody

Immunogen: Human recombinant Neurofilament protein was used as the immunogen. (1)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: The usable concentrations in the Applications Details are suggestions only. The use of different protocols/secondaries/substrates may require the Neurofilament antibody to be titered for optimal performance.

1. The NF421 antibody is specific to the heavy subunit.
2. FFPE tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
3. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Neurofilament antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Neurofilament

Target: Neurofilament

Hintergrund: Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). The fourth neurofilament subunit is alpha internexin in the central nervous system and peripherin in the peripheral nervous system.