MSH6 Antikörper (AA 374-540) (CF®568)

Produktnummer ABIN7657531

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MSH6 in . Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MSH6 Antikörper (AA 374-540) (CF®568) (ABIN7657531)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH6 Antikörper ist konjugiert mit CF®568

Applikation: Bitte anfragen

Klon: MSH6-2111

Produktdetails anti-MSH6 Antikörper (CF®568)

Bindungsspezifität: AA 374-540

Verwendungszweck: MSH6 (DNA Mismatch Repair Protein) (MSH6/2111), CF568 conjugate

Produktmerkmale: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human MSH6 protein (around aa 374-540)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: A431 or HeLa cells. Colon Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Andere Bezeichnung: MSH6

Hintergrund:

Synonyms: DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein

Gene Symbol: MSH6

Molekulargewicht: 85 kDa

Gen-ID: 2956

UniProt: P52701

Pathways: DNA Reparatur, Chromatin Binding, Production of Molecular Mediator of Immune Response