MSH6 Antikörper (AA 374-540)

Produktnummer ABIN6939576

Der Maus Monoklonal anti-MSH6 Antikörper wird verwendet zum Nachweis von MSH6 in Proben von Human. Er wurde validiert für WB, IHC, IF und ELISA.

Kurzübersicht für MSH6 Antikörper (AA 374-540) (ABIN6939576)

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Klon: MSH6-3091

Produktdetails anti-MSH6 Antikörper

Bindungsspezifität: AA 374-540

Verwendungszweck: MSH6 (DNA Mismatch Repair Protein) Antibody

Spezifität: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human MSH6 protein (around aa 374-540) (exact sequence is proprietary)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HCT116, MCF-7 cells, Human colon carcinoma (IHC).

Known Application: ELISA (For coating, order Ab without BSA), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MSH6

Target: MSH6 (MutS Homolog 6 (E. Coli) (MSH6))

Andere Bezeichnung: MSH6

Hintergrund:

DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein

HCT116, Human colon carcinoma (IHC)., MCF-7 cells, HeLa

Molekulargewicht: 163kDa

Gen-ID: 2956, 445052

UniProt: P52701

Pathways: DNA Reparatur, Chromatin Binding, Production of Molecular Mediator of Immune Response