MMP3 Antikörper (AA 317-327) (CF®647)

Produktnummer ABIN7656618

Dieses Anti-MMP3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von MMP3 in WB, IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für MMP3 Antikörper (AA 317-327) (CF®647) (ABIN7656618)

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MMP3 Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 1B4

Produktdetails anti-MMP3 Antikörper (CF®647)

Bindungsspezifität: AA 317-327

Verwendungszweck: MMP3 (Marker of Metastasis and Rheumatoid Arthritis)(1B4), CF647 conjugate

Produktmerkmale: The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment corresponding to amino acids 317-327 of human MMP3.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: Use at 2-4 μg/mL for coating, Order without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: A431 cells. Prostate or Lung Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu MMP3

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Andere Bezeichnung: MMP3

Hintergrund:

Synonyms: CHDS6, Matrix metalloproteinase 3, Matrix metalloproteinase-3, MMP3, Proteoglycanase, SL1, STMY1, STR1, Stromelysin-1, Transin 1

Gene Symbol: MMP3

Molekulargewicht: 57 kDa

Gen-ID: 4314

UniProt: P08254