Rekombinanter IL-10 Antikörper (CF®405S)

Produktnummer ABIN7655640

Dieses Anti-IL-10-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von IL-10 in IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für Rekombinanter IL-10 Antikörper (CF®405S) (ABIN7655640)

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser IL-10 Antikörper ist konjugiert mit CF®405S

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: IL10-2651R

Produktdetails anti-IL-10 Antikörper (CF®405S)

Verwendungszweck: Interleukin 10 (IL10) (Immunoregulation Marker) (IL10/2651R), CF405S conjugate

Produktmerkmale: Interleukin 10 (IL10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immuno-regulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immuno-regulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Interleukin 10 (IL10) protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: SK-BR3 Cells. Adrenal, Lymph Node, Bladder, Prostate or Endometrium.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu IL-10

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Andere Bezeichnung: Interleukin 10

Hintergrund:

Synonyms: Cytokine Synthesis Inhibitory Factor (CSIF), GVHDS, IL-10, IL10, IL10A, Interleukin-10, T-cell Growth Inhibitory Factor (TGIF)

Gene Symbol: IL10

Molekulargewicht: 20 kDa (Monomer), 37 kDa (Dimer)

Gen-ID: 3586

UniProt: P22301

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints