IFNA2 Antikörper (CF®740)

Produktnummer ABIN7655634

Dieses Anti-IFNA2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von IFNA2 in ELISA, WB und Func. Geeignet für Human.

Kurzübersicht für IFNA2 Antikörper (CF®740) (ABIN7655634)

Target: IFNA2 (Interferon, alpha 2 (IFNA2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IFNA2 Antikörper ist konjugiert mit CF®740

Applikation: ELISA, Western Blotting (WB), Functional Studies (Func)

Klon: N27

Produktdetails anti-IFNA2 Antikörper (CF®740)

Verwendungszweck: Interferon alpha-2 (IFNA2) (N27), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein of 16-27 kDa, identified as human interferon-alphaII (IFN-alphaII). Its epitope maps between aa43-53 of the IFN-alphaII) (total aa172). This MAb is specific for IFN-alpha(II) and does not cross-react with IFN-alpha(I). Epitopes of N27 and N39 MAb s are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-alpha(II) after viral infections. The IFN-alpha family consists of 24 or more genes or pseudo-genes. IFN-alphaII) is one of the two distinct families (I and II) of human IFN-alpha. The alpha interferons are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant human IFN-alpha2

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells in 0.1 mL. Immunofluorescence: 0.5-1 μg/mL. Blocking activity order Ab without azide. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Stimulated human peripheral blood mononuclear cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu IFNA2

Target: IFNA2 (Interferon, alpha 2 (IFNA2))

Andere Bezeichnung: Interferon Alpha-2

Hintergrund:

Synonyms: Alpha 2a interferon, IFN alpha, IFN-alpha-2, IFNA, IFNA2, IFNA2B, Interferon alpha 2a, Interferon alpha 2b, Interferon alpha-2, Interferon alpha-A, LeIF2, LeIFA

Gene Symbol: IFNA2

Tissue Expression: Lymphocytes|Monocytes/macrophages

Molekulargewicht: 16-27 kDa

Gen-ID: 3440

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Hepatitis C