Rekombinanter CD74 Antikörper (CF®640R)

Produktnummer ABIN7655129

Der Kaninchen Monoklonal Anti-CD74-Antikörper wurde für FACS, WB, IHC und IHC (fp) validiert. Er ist geeignet, CD74 in Proben von Human, Maus und Pavian zu detektieren.

Kurzübersicht für Rekombinanter CD74 Antikörper (CF®640R) (ABIN7655129)

Target: CD74 (HLA-DR-gamma (CD74))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Pavian

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD74 Antikörper ist konjugiert mit CF®640R

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: CLIP-3127R

Produktdetails anti-CD74 Antikörper (CF®640R)

Verwendungszweck: CD74 (B-Cell Marker)(CLIP/3127R), CF640R conjugate

Produktmerkmale: Recognizes a protein of ~35 kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma ( ). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: SU-DHL-4 lymphoma cells

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: 293T, Daudi or Raji cells. Tonsil or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74

Hintergrund:

Synonyms: CLIP, DHLAG, Gamma chain of class II antigens, HLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, HLADR-gamma (HLADG), Ia antigen-associated invariant chain, la-gamma, Major histocompatibility complex class II invariant chain, MHC HLA-DR gamma chain

Gene Symbol: CD74

Tissue Expression: Antigen presenting cells

Molekulargewicht: 33-41 kDa

Gen-ID: 972

UniProt: P044233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints