Rekombinanter GFAP Antikörper (Biotin)

Produktnummer ABIN7654393

Dieser Maus Monoklonal Antikörper detektiert spezifisch GFAP in WB, IHC, FACS und IHC (fp). Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Rind (Kuh), Schwein, Kaninchen und Huhn.

Kurzübersicht für Rekombinanter GFAP Antikörper (Biotin) (ABIN7654393)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein, Kaninchen, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: RASTRO-789

Produktdetails anti-GFAP Antikörper (Biotin)

Verwendungszweck: GFAP (Astrocyte & Neural Stem Cell Marker) (rASTRO/789), Biotin conjugate

Produktmerkmale: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human GFAP protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.25-0.5 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Brain or Astrocytoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund:

Synonyms: Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

Gene Symbol: GFAP

Tissue Expression: Glia

Molekulargewicht: ~50 kDa

Gen-ID: 2670

UniProt: P14136