EGLN1 Antikörper (AA 1-24) (CF®405S)

Produktnummer ABIN7653365

Der Maus Monoklonal Anti-EGLN1-Antikörper wurde für validiert. Er ist geeignet, EGLN1 in Proben von Human zu detektieren.

Kurzübersicht für EGLN1 Antikörper (AA 1-24) (CF®405S) (ABIN7653365)

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EGLN1 Antikörper ist konjugiert mit CF®405S

Applikation: Bitte anfragen

Klon: 366G-76-3

Produktdetails anti-EGLN1 Antikörper (CF®405S)

Bindungsspezifität: AA 1-24

Verwendungszweck: EGLN1 / PHD2(366G/76/3), CF405S conjugate

Produktmerkmale: This antibody recognises human prolyl hydroxylase 2 (PHD2), a 46 kDa enzyme expressed abundantly in all tissues with the highest expression in testis. Hypoxia inducible factor-1 (HIF-1) is a transcriptional complex, consisting of an alpha and beta subunit, which plays a key role in coordinating the cellular response to hypoxia. During normal oxygen conditions, the alpha subunit of HIF-1 is rapidly degraded, however when hypoxia occurs this degradation is suppressed and HIF-1 activates the transcription of various genes important for survival and adaptation to hypoxia. Prolyl hydroxylase 2 catalyses the hydroxylation of specific prolyl residues within the HIF-1 alpha subunit, thereby targeting this subunit for degradation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Residues 1-24 of PHD2

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: MCF7 cells. Brain or adrenal gland.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu EGLN1

Target: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Andere Bezeichnung: EGLN1

Hintergrund:

Synonyms: Chromosome 1 Open Reading Frame 12 (C1ORF12), DKFZp761F179, ECYT3, Egl 9 family hypoxia inducible factor 1, Egl nine homolog 1, Egln1, HIF Prolyl Hydroxylase 2 (HIF PH2), HIFPH2, HPH2, Hypoxia inducible factor prolyl hydroxylase 2, ORF13, P4H2, PhD2, PNAS 118, PNAS 137, Prolyl hydroxylase domain-containing protein 2, SM20, Zinc finger MYND domain containing protein 6 (ZMYND6)

Gene Symbol: EGLN1

Molekulargewicht: 46 kDa

Gen-ID: 54583

UniProt: Q9GZT9

Pathways: cAMP Metabolic Process, Warburg Effekt