RAD51 Antikörper (AA 1-134)

Produktnummer ABIN7653251

Der Maus Monoklonal Anti-RAD51-Antikörper wurde für IHC, FACS und IHC (fp) validiert. Er ist geeignet, RAD51 in Proben von Human zu detektieren.

Kurzübersicht für RAD51 Antikörper (AA 1-134) (ABIN7653251)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD51 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: RAD51-2702

Produktdetails anti-RAD51 Antikörper

Bindungsspezifität: AA 1-134

Verwendungszweck: RAD51 (Prognostic and Response to Chemotherapy Marker)(RAD51/2702)

Produktmerkmale: RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies. RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human RAD51 protein (around aa 1-134)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: K562, HeLa, MCF-7 cells. Colon or breast Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: RAD51

Hintergrund:

Synonyms: RCA1/BRCA2 containing complex, subunit 5, BRCC5, DNA repair protein RAD51, FANCR, HsT16930, MRMV2, RAD51, RAD51 recombinase, RAD51A, RECA, RecA like protein, Recombination protein A

Gene Symbol: RAD51

Molekulargewicht: 37 kDa

Gen-ID: 5888

UniProt: Q06609

Pathways: DNA Reparatur