RAD51 Antikörper (AA 1-134)

Produktnummer ABIN6940476

Dieser Maus Monoklonal Antikörper detektiert spezifisch RAD51 in IHC und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für RAD51 Antikörper (AA 1-134) (ABIN6940476)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD51 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: RAD51-2702

Produktdetails anti-RAD51 Antikörper

Bindungsspezifität: AA 1-134

Verwendungszweck: RAD51 (Prognostic and Response to Chemotherapy Marker) Antibody

Spezifität: RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies.RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human RAD51 protein (around aa 1-134) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: K562, HeLa, MCF-7 cells. Colon or breast Carcinoma.

Known Application: Flow Cytometry (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: RAD51

Hintergrund:

RCA1/BRCA2 containing complex, subunit 5, BRCC5, DNA repair protein RAD51, FANCR, HsT16930, MRMV2, RAD51, RAD51 recombinase, RAD51A, RECA, RecA like protein, Recombination protein A

HeLa, K562, MCF-7 cells. Colon or breast Carcinoma.

Molekulargewicht: 37kDa

Gen-ID: 5888, 631709

UniProt: Q06609

Pathways: DNA Reparatur