Cyclin E1 Antikörper (AA 10-176)

Produktnummer ABIN7652801

Der Maus Monoklonal Anti-Cyclin E1-Antikörper wurde für WB, IHC und IHC (fp) validiert. Er ist geeignet, Cyclin E1 in Proben von Human zu detektieren.

Kurzübersicht für Cyclin E1 Antikörper (AA 10-176) (ABIN7652801)

Target: Cyclin E1 (CCNE1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin E1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: CCNE1-2460

Produktdetails anti-Cyclin E1 Antikörper

Bindungsspezifität: AA 10-176

Verwendungszweck: Cyclin E (G1/S-Phase Cyclin) (CCNE1/2460)

Produktmerkmale: Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Cyclin E (CCNE1) protein fragment (around aa 10-176)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa Cells. Breast or Colon Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Cyclin E1

Target: Cyclin E1 (CCNE1)

Andere Bezeichnung: Cyclin E

Hintergrund:

Synonyms: CCNE1, Cyclin E1, G1/S-specific cyclin-E1

Gene Symbol: CCNE1

Molekulargewicht: 53 kDa

Gen-ID: 898

Pathways: Zellzyklus, Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Mitotic G1-G1/S Phases