Cyclin B2 Antikörper (CF®647)

Produktnummer ABIN7652745

Dieses Anti-Cyclin B2-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Cyclin B2 in IF. Geeignet für Human, Maus, Ratte und Xenopus laevis.

Kurzübersicht für Cyclin B2 Antikörper (CF®647) (ABIN7652745)

Target: Cyclin B2 (CCNB2)

Reaktivität: Human, Maus, Ratte, Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B2 Antikörper ist konjugiert mit CF®647

Applikation: Immunofluorescence (IF)

Klon: X29-2

Produktdetails anti-Cyclin B2 Antikörper (CF®647)

Verwendungszweck: Cyclin B2(X29.2), CF647 conjugate

Produktmerkmale: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: For coating, order antibody without BSA, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: HeLa cells. Human cervical tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Cyclin B2

Target: Cyclin B2 (CCNB2)

Andere Bezeichnung: Cyclin B2

Hintergrund:

Synonyms: ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694

Gene Symbol: CCNB2

Molekulargewicht: 51 kDa

Gen-ID: 9133

UniProt: O95067

Pathways: Zellzyklus, M Phase