Cyclin B1 Antikörper (CF®740)

Produktnummer ABIN7652735

Der Maus Monoklonal Anti-Cyclin B1-Antikörper wurde für IF und FACS validiert. Er ist geeignet, Cyclin B1 in Proben von Human, Maus und Hamster zu detektieren.

Kurzübersicht für Cyclin B1 Antikörper (CF®740) (ABIN7652735)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B1 Antikörper ist konjugiert mit CF®740

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: V92-1

Produktdetails anti-Cyclin B1 Antikörper (CF®740)

Verwendungszweck: Cyclin B1(V92.1), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Hamster Cyclin B1 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunoprecipitation 1-2 μg/500 μg protein, precipitates active CDK1/cyclin B1 complexes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1

Hintergrund:

Synonyms: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Gene Symbol: CCNB1

Molekulargewicht: 55-62 kDa

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase