Rekombinanter Cyclin B1 Antikörper (CF®405S)

Produktnummer ABIN7652731

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch Cyclin B1 in . Er zeigt eine Reaktivität gegenüber Human, Maus und Hamster.

Kurzübersicht für Rekombinanter Cyclin B1 Antikörper (CF®405S) (ABIN7652731)

Target: Cyclin B1 (CCNB1)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Hamster

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B1 Antikörper ist konjugiert mit CF®405S

Applikation: Bitte anfragen

Klon: CCNB1-2978R

Produktdetails anti-Cyclin B1 Antikörper (CF®405S)

Verwendungszweck: Cyclin B1 (G2- & M-phase Cyclin)(CCNB1/2978R), CF405S conjugate

Produktmerkmale: Recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CCNB1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1

Hintergrund:

Synonyms: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1

Gene Symbol: CCNB1

Molekulargewicht: 55-62 kDa

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase