CD46 Antikörper (CF®740)

Produktnummer ABIN7651623

Dieses Anti-CD46-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD46 in FACS, IHC, Func und IHC (fp). Geeignet für Human und Pavian.

Kurzübersicht für CD46 Antikörper (CF®740) (ABIN7651623)

Target: CD46

Reaktivität: Human, Pavian

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD46 Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 169-1-E4-3

Produktdetails anti-CD46 Antikörper (CF®740)

Verwendungszweck: CD46(169-1-E4.3), CF740 conjugate

Produktmerkmale: Recognizes a protein of 52 kDa-58 kDa, identified as CD46 (also known as membrane cofactor protein, MCP). Its epitope is located on the four immunogenic short consensus repeat (SCR) domains at N-terminus of the protein. CD46 exists as many isoforms in a variety of tissues. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. CD46 functions as a C3b/C4b-binding glycoprotein that inhibits complement activation on host cells. It also serves as a measles virus receptor, an adherence factor for group A Streptococcus pyogenes, and a cellular pilus receptor for pathogenic Neisseria.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Does not react with horse, others not tested. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: K562, U937, HL60, HeLa and Jurkat cells, and human lymphocytes. Lymph node and tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD46

Target: CD46

Andere Bezeichnung: CD46

Hintergrund:

Synonyms: AHUS2, Complement membrane cofactor protein, MCP, Measles virus receptor, Membrane cofactor protein, MIC10, TLX, Trophoblast leucocyte common antigen, Trophoblast lymphocyte cross reactive antigen

Gene Symbol: CD46

Molekulargewicht: 52-58 kDa

Gen-ID: 4179

UniProt: P15529

Pathways: Regulation of Actin Filament Polymerization