PD-L1 Antikörper (AA 39-191) (CF®405S)

Produktnummer ABIN7651046

Der Maus Monoklonal Anti-PD-L1-Antikörper wurde für FACS und IF validiert. Er ist geeignet, PD-L1 in Proben von Human zu detektieren.

Kurzübersicht für PD-L1 Antikörper (AA 39-191) (CF®405S) (ABIN7651046)

Target: PD-L1 (CD274 (PD-L1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PD-L1 Antikörper ist konjugiert mit CF®405S

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: PDL1-2743

Produktdetails anti-PD-L1 Antikörper (CF®405S)

Bindungsspezifität: AA 39-191

Verwendungszweck: PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)(PDL1/2743), CF405S conjugate

Produktmerkmale: PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human CD274 protein (around aa 39-191)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: For coating, order antibody without BSA, Flow cytometry: 1-2 μg/mL, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: Heart, Placenta, Spleen or Tonsil (IHC). Jurkat cells (IF/FACS).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu PD-L1

Target: PD-L1 (CD274 (PD-L1))

Andere Bezeichnung: B7-H1

Hintergrund:

Synonyms: B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1

Gene Symbol: CD274

Molekulargewicht: 37-50 kDa

Gen-ID: 29126

UniProt: Q9NZQ7

Pathways: Cancer Immune Checkpoints