CD22 Antikörper (AA 52-178) (Biotin)

Produktnummer ABIN7651017

Dieses Anti-CD22-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD22 in FACS, WB und IF. Geeignet für Human.

Kurzübersicht für CD22 Antikörper (AA 52-178) (Biotin) (ABIN7651017)

Target: CD22 (CD22 Molecule (CD22))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD22 Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Klon: BLCAM-1796

Produktdetails anti-CD22 Antikörper (Biotin)

Bindungsspezifität: AA 52-178

Verwendungszweck: CD22 / BL-CAM (B-Cell Marker) (BLCAM/1796), Biotin conjugate

Produktmerkmale: This antibody recognizes a protein of 130-140 kDa, identified as CD22 (also known as BL-CAM). CD22 expression is restricted to normal and neoplastic B cells and is absent from other haemopoietic cell types. In B-cell ontogeny, CD22 is first expressed in the cytoplasm of pro-B and pre-B cells, and on the surface as B cells mature to become IgD . It is not expressed by plasma cells, CD22 is found highly expressed in follicular mantle and marginal zone B-cells, and while germinal center B-cells are relatively weak. CD22 is a member of the immunoglobulin superfamily and serves as an adhesion receptor for sialic acid-bearing ligands expressed on erythrocytes and all leukocyte classes. It also associates with tyrosine kinases and play a role in signal transduction and B-cell activation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human CD22 protein (around aa 52-178)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: Raji or Ramos cells (IF/FACS). Raji cell lysate (WB). Human tonsil, lymph node or spleen (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu CD22

Target: CD22 (CD22 Molecule (CD22))

Andere Bezeichnung: BL-CAM

Hintergrund:

Synonyms: B-lymphocyte cell adhesion molecule (BL-CAM), B-cell receptor CD22, CD22, Lectin 2, Lyb8, Sialic acid-binding Ig-like lectin 2 (Siglec-2), SIGLEC2, T-cell surface antigen Leu-14

Gene Symbol: CD22

Tissue Expression: B-cells|Lymphocytes

Molekulargewicht: 130-140 kDa

Gen-ID: 933

UniProt: P20273