Rekombinanter beta Catenin Antikörper

Produktnummer ABIN7650641

Der Kaninchen Monoklonal anti-beta Catenin Antikörper wird verwendet zum Nachweis von beta Catenin in Proben von Human. Er wurde validiert für WB, IHC, FACS, IF und IHC (fp).

Kurzübersicht für Rekombinanter beta Catenin Antikörper (ABIN7650641)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser beta Catenin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: CTNNB1-2030R

Produktdetails anti-beta Catenin Antikörper

Verwendungszweck: Catenin, beta (CTNNB1) (CTNNB1/2030R)

Produktmerkmale: Beta-catenin associates with the cytoplasmic portion of E-cadherin. The catenin/cadherin complexes play an important role mediating cellular adhesion, including adherens junctions. Beta-catenin is involved in the Wnt signaling pathway as well as other signaling pathways, and is also found in complexes with many different proteins including the tumor suppressor protein APC. Defects in beta-catenin are associated with colorectal cancer, as well as many other cancer types. Beta-catenin is normally localized to the cell membrane, but can translocate to the nucleus in response to certain cell signaling. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human i²-catenin protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa or MCF-7 cells. Breast carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Andere Bezeichnung: Beta-catenin

Hintergrund:

Synonyms: Beta-catenin, Catenin beta-1, Catenin (Cadherin associated protein), beta 1, CTNNB

Gene Symbol: CTNNB1

Tissue Expression: Epithelial cells

Molekulargewicht: 92 kDa

Gen-ID: 1499

UniProt: P35222

Pathways: Peptide Hormone Metabolism