BRCA1 Antikörper (AA 445-620) (CF®740)

Produktnummer ABIN7650146

Der Maus Monoklonal Anti-BRCA1-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, BRCA1 in Proben von Human zu detektieren.

Kurzübersicht für BRCA1 Antikörper (AA 445-620) (CF®740) (ABIN7650146)

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BRCA1 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: BRCA1-1398

Produktdetails anti-BRCA1 Antikörper (CF®740)

Bindungsspezifität: AA 445-620

Verwendungszweck: BRCA1 (Breast Marker) (BRCA1/1398), CF740 conjugate

Produktmerkmale: BRCA1 is a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. It combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human BRCA1 protein fragment (aa445-620)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology Formalin-fixed 5-10 μg/mL for 60 min at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: A431 or HeLa Cells. Breast, Ovarian or Renal Cell Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Andere Bezeichnung: BRCA1

Hintergrund:

Synonyms: BRCA1, Breast and ovarian cancer susceptibility protein 1, Early Onset Breast Cancer 1, Protein phosphatase 1 regulatory subunit 53, RING finger protein 53

Gene Symbol: BRCA1

Molekulargewicht: 220 kDa

Gen-ID: 672

UniProt: P38398

Pathways: Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus