Rekombinanter ACTH Antikörper (AA 1-24) (CF®640R)

Produktnummer ABIN7649216

Der Kaninchen Monoklonal Anti-ACTH-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, ACTH in Proben von Human, Ratte, Maus und Diverse Spezies zu detektieren.

Kurzübersicht für Rekombinanter ACTH Antikörper (AA 1-24) (CF®640R) (ABIN7649216)

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Diverse Spezies

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser ACTH Antikörper ist konjugiert mit CF®640R

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: CLIP-2040R

Produktdetails anti-ACTH Antikörper (CF®640R)

Bindungsspezifität: AA 1-24

Verwendungszweck: ACTH (Adrenocorticotrophic Hormone) (Pituitary Marker) (CLIP/2040R), CF640R conjugate

Produktmerkmale: ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH), does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Synthetic peptide corresponding to aa1-24 of human ACTH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Normal pituitary gland or pituitary tumor

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ACTH

Target: ACTH (Adrenocorticotropic hormone (ACTH))

Andere Bezeichnung: ACTH

Hintergrund:

Synonyms: Pro-opiomelanocortin (POMC), Adrenocorticotropin, Adrenocorticotropic hormone, Melanocyte Stimulating Hormone (MSH), Melanotropin, beta-Endorphin, Lipotropin, Met-Enkephalin, POC

Gene Symbol: POMC

Molekulargewicht: ACTH is ~5 kDa, and the POMC precursor is ~30 kDa. The molecular weight of POMC depends upon isoform variation and post-translational modifications.

Gen-ID: 5443

UniProt: P01189

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity