GADD45B Antikörper (N-Term)

Produktnummer ABIN7603219

Der Kaninchen Polyklonal Anti-GADD45B-Antikörper wurde für IHC und WB validiert. Er ist geeignet, GADD45B in Proben von Human zu detektieren.

Kurzübersicht für GADD45B Antikörper (N-Term) (ABIN7603219)

Target: GADD45B (Growth Arrest and DNA-Damage-Inducible, beta (GADD45B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GADD45B Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Produktdetails anti-GADD45B Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-GADD45B Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-GADD45B Antibody Picoband® (ABIN7603219). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GADD45B, which shares 94.7% amino acid (aa) sequence identity with mouse GADD45B.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
1. De Smaele, E., Zazzeroni, F., Papa, S., Nguyen, D. U., Jin, R., Jones, J., Cong, R., Franzoso, G. Induction of gadd45-beta by NF-kappa-B downregulates pro-apoptotic JNK signaling. Nature 414: 308-313, 2001. 2. Ma, D. K., Jang, M.-H., Guo, J. U., Kitabatake, Y., Chang, M., Pow-anpongkul, N., Flavell, R. A., Lu, B., Ming, G., Song, H. Neuronal activity-induced Gadd45b promotes epigenetic DNA demethylation and adult neurogenesis. Science 323: 1074-1077, 2009. 3. Takekawa, M., Saito, H. A family of stress-inducible GADD45-like proteins mediate activation of the stress-responsive MTK1/MEKK4 MAPKKK. Cell 95: 521-530, 1998.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu GADD45B

Target: GADD45B (Growth Arrest and DNA-Damage-Inducible, beta (GADD45B))

Andere Bezeichnung: GADD45B

Hintergrund:

Synonyms: Rhombotin-2, Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2, LMO2, RBTN2, RBTNL1, RHOM2, TTG2

Background: Growth arrest and DNA-damage-inducible, beta, also known as GADD45B, is a protein which in humans is encoded by the GADD45B gene. This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The genes in this group respond to environmental stresses by mediating activation of the p38/JNK pathway. This activation is mediated via their proteins binding and activating MTK1/MEKK4 kinase, which is an upstream activator of both p38 and JNK MAPKs. The function of these genes or their protein products is involved in the regulation of growth and apoptosis. These genes are regulated by different mechanisms, but they are often coordinately expressed and can function cooperatively in inhibiting cell growth.

Molekulargewicht: 18 kDa

Gen-ID: 4616

UniProt: O75293

Pathways: Zellzyklus