PKM Antikörper (N-Term)

Produktnummer ABIN7603151

Dieses Anti-PKM-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PKM in WB, IHC, IF, FACS und ICC. Geeignet für Human.

Kurzübersicht für PKM Antikörper (N-Term) (ABIN7603151)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PKM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 11I4C3

Produktdetails anti-PKM Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-PKM2 Antibody Picoband® (monoclonal, 11I4C3)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PKM2 Antibody Picoband® (monoclonal, 11I4C3) (ABIN7603151). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Ashizawa, K., Willingham, M. C., Liang, C.-M., Cheng, S. In vivo regulation of monomer-tetramer conversion of pyruvate kinase subtype M-2 by glucose is mediated via fructose 1, 6-bisphosphate. 2. J. Biol. Chem. 266: 16842-16846, 1991. 2. Christofk, H. R., Vander Heiden, M. G., Wu, N., Asara, J. M., Cantley, L. C. Pyruvate kinase M2 is a phosphotyrosine-binding protein. 3. Nature 452: 181-186, 2008. 3. Popescu, N. C., Cheng, S. Y. Chromosomal localization of the gene for a human cytosolic thyroid hormone binding protein homologous to the subunit of pyruvate kinase, subtype M(2). Somat. Cell Molec. Genet. 16: 593-598, 1990.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Andere Bezeichnung: PKM

Hintergrund:

Synonyms: Bcl-2 homologous antagonist/killer, Apoptosis regulator BAK, Bcl-2-like protein 7, Bcl2-L-7, BAK1, BAK, BCL2L7, CDN1

Tissue Specificity: Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle.

Background: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds ly and selectively to tyrosine-phosphorylated peptides.

Molekulargewicht: 60 kDa

Gen-ID: 5315

UniProt: P14618

Pathways: Warburg Effekt