CD22 Antikörper (C-Term)

Produktnummer ABIN7602740

Dieses Anti-CD22-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD22 in WB. Geeignet für Human.

Kurzübersicht für CD22 Antikörper (C-Term) (ABIN7602740)

Target: CD22 (CD22 Molecule (CD22))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD22 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 5E7

Produktdetails anti-CD22 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-CD22 Antibody Picoband® (monoclonal, 5E7)

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CD22 Antibody Picoband® (monoclonal, 5E7) (ABIN7602740). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD22, different from the related mouse sequence by ten amino acids.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
1. John, B., Herrin, B. R., Raman, C., Wang, Y., Bobbitt, K. R., Brody, B. A., Justement, L. B. The B cell coreceptor CD22 associates with AP50, a clathrin-coated pit adapter protein, via tyrosine-dependent interaction. J. Immun. 170: 3534-3543, 2003. 2. O'Keefe, T. L., Williams, G. T., Davies, S. L., Neuberger, M. S. Hyperresponsive B cells in CD22-deficient mice. Science 274: 798-801, 1996. 3. Wilson, G. L., Najfeld, V., Kozlow, E., Menniger, J., Ward, D., Kehrl, J. H. Genomic structure and chromosomal mapping of the human CD22 gene. J. Immun. 150: 5013-5024, 1993.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.01 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CD22

Target: CD22 (CD22 Molecule (CD22))

Andere Bezeichnung: CD22

Hintergrund:

Synonyms: B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14, CD22, SIGLEC2

Tissue Specificity: B-lymphocytes.

Background: CD22 is a surface glycoprotein of B lymphocytes that is rapidly phosphorylated on cytoplasmic tyrosines after antigen receptor cross-linking. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. The human CD22 gene is expressed specifically in B lymphocytes and likely has an important function in cell-cell interactions. The B cell coreceptor CD22 plays an important role in regulating signal transduction via the B cell Ag receptor. And CD22 is located within the band region q13.1 of chromosome 19.

Molekulargewicht: 140 kDa

Gen-ID: 933

UniProt: P20273