PIAS2 Antikörper (AA 93-311)

Produktnummer ABIN7602672

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PIAS2 in WB, ELISA und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für PIAS2 Antikörper (AA 93-311) (ABIN7602672)

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIAS2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Produktdetails anti-PIAS2 Antikörper

Bindungsspezifität: AA 93-311

Verwendungszweck: Anti-PIAS2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PIAS2 Antibody Picoband® (ABIN7602672). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PIAS2 recombinant protein (Position: E93-K311).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Liu, B., Liao, J., Rao, X., Kushner, S. A., Chung, C. D., Chang, D. D., Shuai, K. Inhibition of Stat1-mediated gene activation by PIAS1. Proc. Nat. Acad. Sci. 95: 10626-10631, 1998. 2. Tussie-Luna, M. I., Michel, B., Hakre, S., Roy, A. L. The SUMO ubiquitin-protein isopeptide ligase family member Miz1/PIASx-beta/Siz2 is a transcriptional cofactor for TFII-I. J. Biol. Chem. 277: 43185-43193, 2002. 3. Yang, S.-H., Sharrocks, A. D. PIASx-alpha differentially regulates the amplitudes of transcriptional responses following activation of the ERK and p38 MAPK pathways. Molec. Cell 22: 477-487, 2006.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PIAS2

Target: PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))

Andere Bezeichnung: PIAS2

Hintergrund:

Synonyms: E3 SUMO-protein ligase PIAS2, Androgen receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein transferase PIAS2, Msx-interacting zinc finger protein, Miz1, PIAS-NY protein, Protein inhibitor of activated STAT x, Protein inhibitor of activated STAT2, PIAS2, PIASX

Tissue Specificity: Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs.

Background: E3 SUMO-protein ligase PIAS2 is an enzyme that in humans is encoded by the PIAS2 gene. It is mapped to 18q21.1. This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1.

Molekulargewicht: 68 kDa

Gen-ID: 9063

UniProt: O75928

Pathways: JAK-STAT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling