CD74 Antikörper (AA 73-296)

Produktnummer ABIN7602385

Der Kaninchen Polyklonal Anti-CD74-Antikörper wurde für FACS, WB, IF, IHC und ELISA validiert. Er ist geeignet, CD74 in Proben von Human zu detektieren.

Kurzübersicht für CD74 Antikörper (AA 73-296) (ABIN7602385)

Target: CD74 (HLA-DR-gamma (CD74))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CD74 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Produktdetails anti-CD74 Antikörper

Bindungsspezifität: AA 73-296

Verwendungszweck: Anti-CD74 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-CD74 Antibody Picoband® (ABIN7602385). Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human CD74 recombinant protein (Position: Q73-M296).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Claesson, L., Larhammar, D., Rask, L., Peterson, P. A. cDNA clone for the human invariant gamma chain of class II histocompatibility antigens and its implications for the protein structure. Proc. Nat. Acad. Sci. 80: 7395-7399, 1983. 2. Claesson-Welsh, L., Barker, P. E., Larhammar, D., Rask, L., Ruddle, F. H., Peterson, P. A. The gene encoding the human class II antigen-associated gamma chain is located on chromosome 5. Immunogenetics 20: 89-93, 1984. 3. Driessen, C., Bryant, R. A., Lennon-Dumenil, A. M., Villadangos, J. A., Bryant, P. W., Shi, G. P., Chapman, H. A., Ploegh, H. L. Cathepsin S controls the trafficking and maturation of MHC class II molecules in dendritic cells. J. Cell Biol. 147: 775-790, 1999.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Details zu CD74

Target: CD74 (HLA-DR-gamma (CD74))

Andere Bezeichnung: CD74

Hintergrund:

Synonyms: Metabotropic glutamate receptor 5, mGluR5, GRM5, GPRC1E, MGLUR5

Tissue Specificity: Isoform 1 and isoform 2 are detected in bone marrow cells, spermatogonia and spermatocytes, but not in round spermatids, elongating spermatids and spermatozoa. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa, but not in spermatogonia and spermatocytes (at protein level). Isoform 1 is widely expressed and detected in fetal liver and bone marrow. Isoform 3 is detected in bone marrow cells enriched in hematopoietic stem cells.

Background: HLA class II histocompatibility antigen gamma chain also known as HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74), is a protein that in humans is encoded by the CD74 gene. The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

Molekulargewicht: 31-34 kDa

Gen-ID: 972

UniProt: P04233

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints